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Activity 1 |
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NAME_____________________________________PERIOD________ DATE_____________________
IST IT DNA? IST IT HUMAN? WORKSHEET
Activity 1.a
PCR
- Describe the purpose of the polymerase chain reaction (PCR) process:
- Each cycle consists of three main
phases. List and describe each event:
- Why is it necessary to heat the DNA?
- How many copies of DNA are present after 30 PCR cycles?
Activity 1.b
SEQUENCING
- The following steps are involved in
the sequencing of DNA using the Sanger method. Place the
following events in the correct sequence from first to
last.
- TAZ polymerase binds
- Electrophoresis
- DNA denatures
- The simulated gel is read from bottom to top starting with the smallest fragments
- Primers anneal
- Deoxynucleotides and dideoxynucleotides are added
- Laser beam detects color emitted from each dideoxynucleotide as fragments migrate through the gel
- Sequencing reaction loaded onto lane of polyacrylamide gel
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2. What role do dideoxynucleotides play in this procedure?
