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If there is DNA in your sample, it will be first be isolated from the other substances through an extraction procedure. This is the first step in the analysis process.
After DNA has been isolated from a tissue sample, the amount of DNA in the sample must be amplified before its nucleotide sequence can be determined. During the amplification process, the DNA is replicated by a technique called polymerase chain reaction (PCR).
When a substantial amount of DNA has been produced using the PCR technique, it is possible to determine nucleotide sequences in the DNA through the use of the cycle sequencing.
FOLLOW THE DIRECTIONS BELOW TO COMPLETE ACTIVITY 1:
- Print Activity 1 Worksheet in Show Your Stuff.
- Read about the extraction process by visiting the extraction link above.
- Visit the animated polymerase chain reaction site above and complete the Activity 1.a Worksheet.
- Read about DNA sequencing at the FBI. Then visit the animated cycle sequencing site above and complete the Activity 1.b Worksheet.
- Submit the worksheet for the above activities to your teacher and proceed on to Activity 2.
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