This is a fairly advanced lesson in biotechnology, DNA sequencing, and gene expression. Therefore, there is prerequisite information students should know before beginning this lesson. They should understand the genetic code and its relationship to RNA and amino sequence in proteins. An understanding of protein structure and function and the impact of mutations on protein structure would also be useful. The first part of the lesson may actually be the most difficult. However, students should be able to learn about PCR and cycle sequencing on-line. The FBI sites provide excellent background. If students read about these techniques at the FBI prior to playing the animations, they should be well prepared to complete the worksheets. Visit these sites prior to assigning this lesson to your students. If you feel the information is too difficult for them, plan want to walk them through it step-by-step and elaborate as you go. You can either accomplish this alone with a projector or in a computer lab environment.
The animations require Shockwave and Realplayer. To check your system and download Shockwave and Realplayer go to the DNA Learning Center and click on DNA from the Beginning. On this page, you can click to check software and also download Shockwave and Realplayer for free.
DATA BASE DIRECTIONS
Students should copy each nucleotide sequence before entering the data base. At the Blast search page, the sequence can be pasted into the data entry box. Click "Search," then "Format Results." Students will then scroll down the results page. The results will show the nucleotide matches--both species and protein information is give. Students should analyze these results and draw conclusions based on the data provided.
Date Last Modified: 8/29/99